α-黑素细胞刺激素作为人类肥大细胞分泌功能的选择性诱导剂。

alpha-Melanocyte stimulating hormone acts as a selective inducer of secretory functions in human mast cells.

作者信息

Grützkau A, Henz B M, Kirchhof L, Luger T, Artuc M

机构信息

Experimental Dermatology, Charité, Campus Virchow-Klinikum, Medizinische Fakultät der Humboldt Universität zu Berlin, Augustenburger Platz 1, 13353 Berlin, Germany.

出版信息

Biochem Biophys Res Commun. 2000 Nov 11;278(1):14-9. doi: 10.1006/bbrc.2000.3764.

DOI:10.1006/bbrc.2000.3764

PMID:11071848

Abstract

In the present study, we have investigated the pro-opiomelanocortin (POMC)-derived neuropeptide alpha-MSH for its ability to modulate activation of human mast cells. The in vitro ability of purified human skin mast cells to secrete various types of mast cell mediators was monitored in response to alpha-MSH at the mRNA and at the protein level. Picomolar concentrations of alpha-MSH induced a dose-dependent release of histamine from isolated human skin mast cells and from skin punch biopsies. However, no effect of alpha-MSH was seen regarding the expression of IL-1, IL-6, IL-8, TGF-beta, and TNF-alpha. Melanocortin receptor MC-1 was identified at the transcriptional level by RT-PCR analysis but not at the protein level, whereas, in leukemic human mast cells (HMC-1), the mRNAs and the proteins for the MC-1 and MC-5 receptor were identified. These results suggest that alpha-MSH may selectively induce acute inflammatory effects via secretion of histamine.

摘要

在本研究中，我们研究了源自阿片-促黑素细胞皮质素原（POMC）的神经肽α-促黑素（α-MSH）调节人肥大细胞激活的能力。在mRNA和蛋白质水平上，监测纯化的人皮肤肥大细胞分泌各种类型肥大细胞介质的体外能力，以响应α-MSH。皮摩尔浓度的α-MSH诱导组胺从分离的人皮肤肥大细胞和皮肤打孔活检组织中呈剂量依赖性释放。然而，未观察到α-MSH对白细胞介素-1（IL-1）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、转化生长因子-β（TGF-β）和肿瘤坏死因子-α（TNF-α）表达的影响。通过逆转录-聚合酶链反应（RT-PCR）分析在转录水平鉴定出黑皮质素受体MC-1，但在蛋白质水平未鉴定出；而在人白血病肥大细胞（HMC-1）中，鉴定出了MC-1和MC-5受体的mRNA和蛋白质。这些结果表明，α-MSH可能通过组胺分泌选择性地诱导急性炎症反应。