淡水河豚鲀属毒素的迟发性溶血活性。

Delayed haemolytic activity by the freshwater puffer Tetraodon sp. toxin.

作者信息

Taniyama S, Mahmud Y, Tanu M B, Takatani T, Arakawa O, Noguchi T

机构信息

Graduate School of Science and Technology, Nagasaki University, 1-14, Bunkyo-machi, 852-8021, Nagasaki, Japan.

出版信息

Toxicon. 2001 May;39(5):725-7. doi: 10.1016/s0041-0101(00)00197-5.

DOI:10.1016/s0041-0101(00)00197-5

PMID:11072053

Abstract

In order to elucidate the toxin composition of the freshwater puffer in Bangladesh, about 230 specimens of Tetraodon sp. were collected from 1997 to 1999 and extracted. After partitioning the toxins between an aqueous layer and a 1-butanol layer, the toxin in the aqueous layer was characterized as paralytic shellfish poison (PSP) (data not shown), while the toxin in the 1-butanol layer was identified as palytoxin (PTX) or PTX-like substance based on the delayed haemolytic activity which was inhibited by an anti-PTX antibody and ouabain (g-strophanthin). This is the first report on the occurrence of PTX or PTX-like substance(s) in puffer fish.

摘要

为阐明孟加拉国淡水河豚的毒素成分，1997年至1999年期间收集了约230份圆斑东方鲀标本并进行提取。在将毒素在水层和正丁醇层之间进行分配后，水层中的毒素被鉴定为麻痹性贝类毒素（PSP）（数据未显示），而正丁醇层中的毒素基于被抗多环珊瑚毒素抗体和哇巴因（毒毛花苷）抑制的延迟溶血活性被鉴定为多环珊瑚毒素（PTX）或类多环珊瑚毒素物质。这是关于河豚中出现PTX或类PTX物质的首次报道。