Kain K H, Popov V L, Herzog N K
Department of Pathology, The WHO Collaborating Center for Tropical Diseases, The University of Texas Medical Branch, 301 University Avenue, Galveston, TX 77555-0609, USA.
Biochim Biophys Acta. 2000 Nov 15;1494(1-2):91-103. doi: 10.1016/s0167-4781(00)00223-2.
Stimulation of immune cells results in altered cell function and metabolism, which must be recognized by and coordinated with energy production from mitochondria. Mitochondria contain their own DNA genome encoding 13 polypeptides that combine with nuclear-derived subunits to create functional enzyme complexes of the electron transport chain. Therefore, coordination of mitochondrial and nuclear transcription is necessary to achieve a sustained elevation in mitochondrial ATP production. Pre-B-lymphocytes stimulated with lipopolysaccharide exhibit increased activity levels of the mitochondrial enzymes, succinate dehydrogenase and cytochrome c oxidase. Immunoblot analyses of purified mitochondria indicate an increase in the mitochondrial transcription factor (mtTFA) levels in mitochondria induced by cell stimulation. This increase is consistent with increased mtTFA production in the cytoplasm. In addition, mitochondrial protein extracts indicate an increase in protein binding to a mtTFA-DNA binding site from the mitochondrial genome, subsequent to cell stimulation. These results indicate that mitochondrial activity changes during B-lymphocyte stimulation, and mtTFA may contribute to the coordination of respiration with cellular energy demand.
免疫细胞的刺激会导致细胞功能和代谢的改变，而这必须被线粒体产生能量的过程所识别并与之协调。线粒体含有自己的DNA基因组，可编码13种多肽，这些多肽与细胞核衍生的亚基结合，形成电子传递链的功能性酶复合物。因此，线粒体和细胞核转录的协调对于实现线粒体ATP产生的持续升高是必要的。用脂多糖刺激前B淋巴细胞会使线粒体酶琥珀酸脱氢酶和细胞色素c氧化酶的活性水平增加。对纯化线粒体的免疫印迹分析表明，细胞刺激诱导的线粒体中线粒体转录因子（mtTFA）水平增加。这种增加与细胞质中mtTFA产量的增加一致。此外，线粒体蛋白提取物表明，细胞刺激后，与线粒体基因组中mtTFA-DNA结合位点结合的蛋白质增加。这些结果表明，B淋巴细胞刺激过程中线粒体活性发生变化，mtTFA可能有助于呼吸与细胞能量需求的协调。
