Saso L, Tommasino P, Italiano G, Grippa E, Leone M G, Gatto M T, Silvestrini B
Department of Pharmacology of Natural Substances and General Physiology, University of Rome, Italy.
Physiol Res. 2000;49(4):403-9.
Quantitative and qualitative changes of serum proteins, apart from glycation, have not been sufficiently studied in streptozotocin-induced diabetic rats (D), the most common experimental model for diabetes. Thus, we decided to analyze the serum of diabetic rats by concanavalin A-blotting in comparison with rats with acute inflammation induced by fermented yeast (Y), in which characteristic alterations of serum proteins have been described. Two months after the streptozotocin treatment, the blood glucose levels were highly elevated (456+/-24 vs. 124+/-10 mg/dl, p<0.001, n=12), the body weight was significantly lower than normal (279+/-10 vs. 392+/-6 g, p<0.001, n=12), and serum proteins appeared to be highly glycated (p<0.001) when analyzed by the fructosamine assay, without any significant change in the total serum protein concentration. Analysis by concanavalin A-blotting, revealed a significant decrease of alpha1-inhibitor-3 (alpha1-I3, p<0.05) and an increase of the beta chain of haptoglobin (beta-Hp, p<0.05) in both D and Y rats (n=3) compared with control animals. However, acute inflammation caused a marked rise of two prominent acute phase proteins, alpha2-macroglobulin and hemopexin, which did not change appreciably in diabetic rats. Further work will be necessary to evaluate the physiopathological significance of these phenomena which could result from changes of both concentration and glycosylation of the aforementioned proteins.
除糖基化外，链脲佐菌素诱导的糖尿病大鼠（D组）血清蛋白的定量和定性变化尚未得到充分研究，而糖尿病大鼠是最常见的糖尿病实验模型。因此，我们决定通过伴刀豆球蛋白A印迹法分析糖尿病大鼠的血清，并与发酵酵母诱导的急性炎症大鼠（Y组）进行比较，在Y组中已描述了血清蛋白的特征性改变。链脲佐菌素治疗两个月后，血糖水平显著升高（456±24 vs. 124±10 mg/dl，p<0.001，n=12），体重显著低于正常水平（279±10 vs. 392±6 g，p<0.001，n=12），用果糖胺测定法分析时，血清蛋白似乎高度糖基化（p<0.001），而血清总蛋白浓度无显著变化。伴刀豆球蛋白A印迹法分析显示，与对照动物相比，D组和Y组大鼠（n=3）的α1-抑制因子-3显著降低（α1-I3，p<0.05），触珠蛋白β链增加（β-Hp，p<0.05）。然而，急性炎症导致两种主要急性期蛋白α2-巨球蛋白和血红素结合蛋白显著升高，而在糖尿病大鼠中这些蛋白没有明显变化。有必要进一步开展工作来评估这些现象的生理病理学意义，这些现象可能是由上述蛋白质的浓度和糖基化变化引起的。
