Hisasue M, Nishigaki K, Katae H, Yuri K, Mizuno T, Fujino Y, Setoguchi A, Hasegawa A, Watari T, Masuda K, Ohno K, Tsujimoto H
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
J Vet Med Sci. 2000 Oct;62(10):1059-65. doi: 10.1292/jvms.62.1059.
The clonality analysis of the bone marrow cells was carried out by detecting the integrated proviruses of feline leukemia virus (FeLV) to understand the pathogenesis of FeLV-associated hematopoietic disorders in cats. Bone marrow cells from 4 cases with acute myeloid leukemia (AML), 9 cases with myelodysplastic syndromes (MDS), 2 cases with pure red cell aplasia (PRCA) and 3 healthy carriers infected with FeLV were subjected to Southern blot analyses using an exogenous FeLV probe. Clonal hematopoiesis was found in all the cases with AML and in 6 of the 9 cases with MDS, but not in the cases with both PRCA and healthy carriers infected with FeLV. In the 2 cases with MDS, it was thought that the same clones of the hematopoietic cells might proliferate before and after the progression of the disease irrespective of the changes of the hematological diagnoses by cytological examination. This study indicates that MDS in cats is a disease manifestation as a result of clonal proliferation of hematopoietic cells and can be recognized as a pre-leukemic state of AML.
通过检测猫白血病病毒（FeLV）的整合前病毒，对骨髓细胞进行克隆性分析，以了解猫FeLV相关造血系统疾病的发病机制。对4例急性髓系白血病（AML）、9例骨髓增生异常综合征（MDS）、2例纯红细胞再生障碍性贫血（PRCA）以及3例感染FeLV的健康携带者的骨髓细胞，使用外源性FeLV探针进行Southern印迹分析。在所有AML病例以及9例MDS病例中的6例中发现了克隆性造血，但在PRCA病例和感染FeLV的健康携带者中未发现。在2例MDS病例中，认为无论细胞学检查的血液学诊断如何变化，造血细胞的相同克隆可能在疾病进展前后增殖。本研究表明，猫的MDS是造血细胞克隆增殖的一种疾病表现，可被视为AML的白血病前期状态。
Zotero 插件