Hasegaw T, Sato F, Ishida N, Fukushima Y, Mukoyama H
Laboratory of Molecular and Cellular Biology, Equine Research Institute, Japan Racing Association, Utsunomiya.
J Vet Med Sci. 2000 Oct;62(10):1109-10. doi: 10.1292/jvms.62.1109.
A quick method for sex determination of horses was developed. Simultaneous amplification of the equine sex-determining region of the Y chromosome gene (SRY) and amelogenin gene (AMEL) accomplished the determination of the presence of both the Y chromosome and SRY gene. In agarose gel electrophoresis, a normal stallion showed 1 SRY band and 3 AMEL (AMELX, AMELY, and AMELX/AMELY heteroduplex) bands, and a normal mare showed a single AMELX band. In XY-mares, 3 AMEL bands were detected as in a normal stallion, but no SRY band. The present method enables a quick diagnosis for XY-mare prior to cytogenetic analysis.
开发了一种快速确定马性别的方法。通过同时扩增Y染色体基因（SRY）的马性别决定区域和牙釉蛋白基因（AMEL）来确定Y染色体和SRY基因的存在。在琼脂糖凝胶电泳中，正常种马显示1条SRY带和3条AMEL带（AMELX、AMELY以及AMELX/AMELY异源双链体），正常母马显示1条单一的AMELX带。在XY母马中，检测到与正常种马一样的3条AMEL带，但没有SRY带。本方法能够在细胞遗传学分析之前对XY母马进行快速诊断。
