Ubol Sukathida, Kasisith Jitra
Department of Microbiology, Faculty of Science, Mahidol University, Rama VI Road, Bangkok, Thailand.
J Med Microbiol. 2000 Nov;49(11):1043-1046. doi: 10.1099/0022-1317-49-11-1043.
A laboratory strain of rabies virus has been reported to induce apoptosis in experimental animals. The present study demonstrated that a bat strain and a primary canine rabies virus isolate also induced apoptosis in vivo. This death process involved reactivation of the caspase gene, Nedd-2, a developmentally down-regulated apoptotic gene. Expression of Nedd-2 was significantly up-regulated in infected adult and suckling mice. Reactivation of Nedd-2 in infected adult mice started at around day 3 and was prominent on day 5. The level of expression was constantly high up to the time that mice showed signs of illness. Expression of Nedd-2 correlated with the appearance of apoptotic nuclei within the infected brain, suggesting that reactivation of a developmentally down-regulated gene, Nedd-2, may be required for apoptotic elimination of cells damaged by infection.
据报道，一种实验室毒株的狂犬病病毒可在实验动物中诱导细胞凋亡。本研究表明，一种蝙蝠毒株和一种犬源狂犬病病毒原始分离株在体内也可诱导细胞凋亡。这一死亡过程涉及半胱天冬酶基因Nedd-2的重新激活，Nedd-2是一种在发育过程中表达下调的凋亡基因。在受感染的成年小鼠和乳鼠中，Nedd-2的表达显著上调。受感染成年小鼠中Nedd-2的重新激活在第3天左右开始，并在第5天变得明显。在小鼠出现疾病迹象之前，其表达水平一直很高。Nedd-2的表达与受感染大脑中凋亡细胞核的出现相关，这表明重新激活发育过程中表达下调的基因Nedd-2可能是凋亡清除受感染损伤细胞所必需的。
