棕色固氮菌mucA和mucC基因产物在藻酸盐生产中的作用。
Role of Azotobacter vinelandii mucA and mucC gene products in alginate production.
作者信息
Núñez C, León R, Guzmán J, Espín G, Soberón-Chávez G
机构信息
Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62251, Mexico.
出版信息
J Bacteriol. 2000 Dec;182(23):6550-6. doi: 10.1128/JB.182.23.6550-6556.2000.
Abstract
Azotobacter vinelandii produces the exopolysaccharide alginate, which is essential for its differentiation to desiccation-resistant cysts. In different bacterial species, the alternative sigma factor sigma(E) regulates the expression of functions related to the extracytoplasmic compartments. In A. vinelandii and Pseudomonas aeruginosa, the sigma(E) factor (AlgU) is essential for alginate production. In both bacteria, the activity of this sigma factor is regulated by the product of the mucA, mucB, mucC, and mucD genes. In this work, we studied the transcriptional regulation of the A. vinelandii algU-mucABCD gene cluster, as well as the role of the mucA and mucC gene products in alginate production. Our results show the existence of AlgU autoregulation and show that both MucA and MucC play a negative role in alginate production.
摘要
棕色固氮菌产生胞外多糖藻酸盐，这对其分化为抗干燥囊肿至关重要。在不同的细菌物种中，替代西格玛因子西格玛（E）调节与细胞外区室相关功能的表达。在棕色固氮菌和铜绿假单胞菌中，西格玛（E）因子（AlgU）对藻酸盐的产生至关重要。在这两种细菌中，该西格玛因子的活性受mucA、mucB、mucC和mucD基因产物的调节。在这项工作中，我们研究了棕色固氮菌algU-mucABCD基因簇的转录调控，以及mucA和mucC基因产物在藻酸盐产生中的作用。我们的结果表明存在AlgU自我调节，并表明MucA和MucC在藻酸盐产生中均起负作用。
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