Acrosomal enzymes: Immunochemical localization of acrosin and hyaluronidase in ram spermatozoa.

Acrosin and hyaluronidase have been localized in the acrosomal region of ram spermatozoa using specific antibodies raised against the highly purified enzymes. Hyaluronidase staining was denser at the periphery of the sperm head; whereas acrosin staining was denser in the equatorial region and appeared to be bound to the inner acrosomal membrane.