Hafez H M, Hess M, Prusas C, Naylor C J, Cavanagh D
Institute of Poultry Diseases, Free University of Berlin, Germany.
J Vet Med B Infect Dis Vet Public Health. 2000 Oct;47(8):629-33. doi: 10.1046/j.1439-0450.2000.00398.x.
Three isolates of avian pneumovirus (APV) were isolated in Germany during 1987 and 1988 from turkeys with clinical signs of turkey rhinotracheitis and one was isolated during 1990 from a broiler breeder flock with typical signs of swollen head syndrome. The isolates were typed using type-specific reverse transcriptase-polymerase chain reactions. The three isolates from turkeys were identified as type A, while the isolate from the broiler breeders was type B. During the late 1980s no APV live-virus vaccine was used in poultry flocks in Germany, which is indicative of the presence of both types at that time. Previous isolates detected from elsewhere in Europe during the 1980s had been only of type B.
1987年至1988年期间,德国从出现火鸡鼻气管炎临床症状的火鸡中分离出三株禽肺病毒(APV),1990年从一个出现典型肿头综合征症状的肉种鸡群中分离出一株。使用型特异性逆转录聚合酶链反应对分离株进行分型。从火鸡中分离出的三株为A型,而从肉种鸡中分离出的为B型。20世纪80年代后期,德国的家禽群未使用APV活病毒疫苗,这表明当时两种类型都存在。20世纪80年代在欧洲其他地方检测到的先前分离株仅为B型。