通过1H-15N NMR HMBC实验证明，在低pH值下Zn2+在ATP的N1位点结合，在高pH值下在N7位点结合。

Binding site of Zn2+ in ATP:N1 at low pH and N7 at high pH as evidenced by 1H-15N NMR HMBC experiments.

作者信息

Du F, Mao X A

机构信息

Laboratory of Magnetic Resonance and Atomic Molecular Physics, Wuhan Institute of Physics and Mathematics, The Chinese Academy of Sciences, People's Republic of China.

出版信息

Spectrochim Acta A Mol Biomol Spectrosc. 2000 Nov 1;56A(12):2391-5. doi: 10.1016/s1386-1425(00)00286-9.

DOI:10.1016/s1386-1425(00)00286-9

PMID:11075679

Abstract

Nuclear magnetic resonance (NMR) 1H-15N heteronuclear multiple bond correlation (HMBC) experiments on natural abundant adenosine-5'-triphosphate (ATP) showed that zinc(II) induced large chemical shifts (> 10 ppm) for N1 at lower pH (2-5) while for N7 at higher pH (5-7), suggesting that the binding site of Zn2+ in the purine base of ATP is pH dependent. The size effect of zinc(II) in coordination is also discussed.

摘要

对天然丰度的腺苷 - 5'-三磷酸（ATP）进行的核磁共振（NMR）1H - 15N 异核多键相关（HMBC）实验表明，在较低pH值（2 - 5）时，锌（II）会使N1产生较大的化学位移（> 10 ppm），而在较高pH值（5 - 7）时会使N7产生较大化学位移，这表明ATP嘌呤碱基中Zn2 + 的结合位点依赖于pH值。同时还讨论了锌（II）配位中的尺寸效应。