Steinbac O C, Wolffe A P, Rupp R A
Friedrich-Miescher-Laboratorium der Max-Planck-Gesellschaft, Tübingen, Germany.
Biol Chem. 2000 Sep-Oct;381(9-10):1013-6. doi: 10.1515/BC.2000.124.
Acetylation of nucleosome core histones, which is positively correlated with transcriptional activity, is developmentally regulated in Xenopus. Here we have used the specific histone deacetylase (HDAC)-inhibitor trichostatin A (TSA) to induce precocious histone hyperacetylation in the early frog embryo in order to investigate the potential role of the endogenous changes in chromatin acetylation for the temporally programmed induction of skeletal myogenesis. We show that TSA-treatment (i) selectively blocked the transcriptional induction of the myoD gene, and (ii) severely reduced subsequent muscle differentiation. Both phenotypes required TSA application before gastrulation. This indicates that HDAC activity is required early for the formation of the frog embryonic musculature, apparently for the induction of the MyoD-dependent muscle cell lineage.
核小体核心组蛋白的乙酰化与转录活性呈正相关，在非洲爪蟾中其受发育调控。在此，我们使用特异性组蛋白去乙酰化酶（HDAC）抑制剂曲古抑菌素A（TSA）诱导早期蛙胚过早发生组蛋白高度乙酰化，以研究染色质乙酰化内源性变化在骨骼肌生成的时间程序性诱导中的潜在作用。我们发现，TSA处理（i）选择性地阻断了myoD基因的转录诱导，并且（ii）严重降低了随后的肌肉分化。这两种表型都需要在原肠胚形成之前应用TSA。这表明HDAC活性在蛙胚胎肌肉组织形成的早期是必需的，显然对于MyoD依赖的肌肉细胞谱系的诱导是必需的。
