Lim J, Jang B, Lee R, Park S, Yun H
Division of Veterinary Pharmacology and Toxicology, Chungnam National University, Taejon, South Korea.
J Chromatogr B Biomed Sci Appl. 2000 Sep 15;746(2):219-25. doi: 10.1016/s0378-4347(00)00334-0.
A highly sensitive and specific method for the determination of roxithromycin in the flounder muscle by LC-MS was developed and validated. A dichloromethane extract of the sample was separated on C18 reversed-phase column with acetonitrile-50 mM ammonium acetate (80:20, v/v) as the mobile phase and analyzed by LC-MS via atmospheric pressure ionization/electrospray ionization interface. The limit of detection and limit of quantitation were 0.01 and 0.1 ng/g, respectively. Mean recoveries from spiked muscles were 81.1% (ranged from 71.0 to 90.3%) for roxithromycin. The method has been successfully applied to determine roxithromycin in the flounder muscle.
建立并验证了一种通过液相色谱-质谱法测定比目鱼肌肉中罗红霉素的高灵敏度和特异性方法。样品的二氯甲烷提取物在C18反相柱上进行分离，以乙腈-50 mM醋酸铵（80:20，v/v）作为流动相，并通过大气压电离/电喷雾电离接口的液相色谱-质谱法进行分析。检测限和定量限分别为0.01和0.1 ng/g。罗红霉素在加标肌肉中的平均回收率为81.1%（范围为71.0%至90.3%）。该方法已成功应用于测定比目鱼肌肉中的罗红霉素。
