Yanagihara Y, Ohtani M, Kariya S, Uchino K, Aoyama T, Yamamura Y, Iga T
Department of Hospital Pharmacy, Tokyo Posts and Telecommunications Hospital, Japan.
J Chromatogr B Biomed Sci Appl. 2000 Sep 15;746(2):227-31. doi: 10.1016/s0378-4347(00)00331-5.
A stereoselective high-performance liquid chromatographic method for the determination of the enantiomers of ketamine and its active metabolite, norketamine, in human plasma is described. The compounds were extracted from plasma by liquid-liquid extraction three times in a combination of cyclohexane with 2.5 M NaOH, 1 mM HCl and 1 M carbonate buffer. Stereoselective separation was achieved on a Chiralcel OD column with a mobile phase of n-hexane-2-propanol (98:2, v/v). The detection wavelength was 215 nm. The lower limits of the determination of the method were 5 ng/ml for ketamine and 10 ng/ml for norketamine. The intra- and inter-day coefficients of variation ranged from 2.9 to 9.8% and from 3.4 to 10.7% for all compounds, respectively. The method was sensitive and sufficiently reproducible for stereoselective monitoring of ketamine and norketamine in human plasma during pharmacokinetic studies after the administration of ketamine for analgesia.
本文描述了一种用于测定人血浆中氯胺酮及其活性代谢物去甲氯胺酮对映体的立体选择性高效液相色谱法。通过液液萃取，用环己烷与2.5 M氢氧化钠、1 mM盐酸和1 M碳酸盐缓冲液的组合从血浆中提取化合物三次。在Chiralcel OD柱上，以正己烷-2-丙醇（98:2，v/v）为流动相实现立体选择性分离。检测波长为215 nm。该方法的测定下限为氯胺酮5 ng/ml，去甲氯胺酮10 ng/ml。所有化合物的日内和日间变异系数分别为2.9%至9.8%和3.4%至10.7%。该方法灵敏且具有足够的重现性，可用于在氯胺酮镇痛给药后的药代动力学研究中对人血浆中的氯胺酮和去甲氯胺酮进行立体选择性监测。
