Wróbel M, Czubak J
Institute of Medical Biochemistry, Collegium Medicum UJ, Kraków, Poland.
J Chromatogr B Biomed Sci Appl. 2000 Sep 15;746(2):315-8. doi: 10.1016/s0378-4347(00)00336-4.
The molecular mass of rhodanese from the mitochondrial fraction of frog Rana temporaria liver, equaling 8.7 kDa, was determined by high-performance size exclusion chromatography (HP-SEC). The considerable difference in molecular weight and the lack of common antigenic determinants between frog liver rhodanese and bovine rhodanese suggest the occurrence of different forms of this sulfurtransferase in the liver of these animals.
通过高效尺寸排阻色谱法(HP-SEC)测定了来自青蛙(林蛙)肝脏线粒体部分的硫氰酸酶的分子量,其为8.7 kDa。青蛙肝脏硫氰酸酶与牛硫氰酸酶在分子量上存在显著差异,且缺乏共同的抗原决定簇,这表明在这些动物的肝脏中存在这种硫转移酶的不同形式。