Turner D J, cowles R A, Segura B J, Romanchuk G, Barnhart D C, Mulholland M W
Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI, USA.
J Gastrointest Surg. 2000 Sep-Oct;4(5):475-80. doi: 10.1016/s1091-255x(00)80089-x.
Pancreatic exocrine function has been demonstrated to be under neuronal regulation. The pathways responsible for this effect, and the long-term consequences of such interactions, are incompletely described. The effects of neuronal depolarization on pancreatic acinar cells were studied to determine whether calcium signaling and c-fos expression were activated. In pancreatic lobules, which contain both neurons and acinar cells, agonists that selectively stimulated neurons increased intracellular calcium in acinar cells. Depolarization also led to the expression of c-fos protein in 24% +/- 4% of the acinar cells. In AR42J pancreatic acinar cells, cholinergic stimulation demonstrated an average increase of 398 +/- 19 nmol/L in intracellular calcium levels, and induced c-fos expression that was time and dose dependent. The data indicate that intrapancreatic neurons induce Ca²+ signaling and early-response gene expression in pancreatic acinar cells.
胰腺外分泌功能已被证明受神经调节。负责这种效应的途径以及这种相互作用的长期后果尚未完全阐明。研究了神经元去极化对胰腺腺泡细胞的影响，以确定钙信号传导和c-fos表达是否被激活。在同时含有神经元和腺泡细胞的胰腺小叶中，选择性刺激神经元的激动剂会增加腺泡细胞内的钙含量。去极化还导致24%±4%的腺泡细胞中c-fos蛋白表达。在AR42J胰腺腺泡细胞中，胆碱能刺激显示细胞内钙水平平均增加398±19 nmol/L，并诱导了时间和剂量依赖性的c-fos表达。数据表明胰腺内神经元可诱导胰腺腺泡细胞中的Ca²⁺信号传导和早期反应基因表达。
