氨基甲酰磷酸对柱状鱼腥藻中固氮酶的影响
Effect of carbamoyl phosphate on nitrogenase in Anabaena cylindrica Lemm.
作者信息
Lawrie A C
出版信息
J Bacteriol. 1979 Jul;139(1):115-9. doi: 10.1128/jb.139.1.115-119.1979.
Abstract
Carbamoyl phosphate inhibited acetylene reduction by whole cells and cell-free extracts of Anabaena cylindrica. Higher levels of both endogenous carbamoyl phosphate and carbamoyl phosphate synthase activity were present in NH4+-grown cells (in which acetylene reduction was absent) than in N2-grown cells (in which acetylene reduction was present). However, inhibition of acetylene reduction was observed also with cyanate, the main initial decomposition product under the conditions used. It is concluded that carbamoyl phosphate or one of its metabolites may act as a physiological regulator of both nitrogenase activity and synthesis, but caution must be used in interpreting effects observed several hours after the addition of carbamoyl phosphate, because the effects may be due to cyanate.
摘要
氨甲酰磷酸抑制了圆柱鱼腥藻全细胞和无细胞提取物的乙炔还原反应。与生长在氮气中的细胞（存在乙炔还原反应）相比，生长在铵离子中的细胞（不存在乙炔还原反应）内源性氨甲酰磷酸和氨甲酰磷酸合成酶活性水平更高。然而，在所使用的条件下，作为主要初始分解产物的氰酸盐也能抑制乙炔还原反应。由此得出结论，氨甲酰磷酸或其一种代谢产物可能作为固氮酶活性和合成的生理调节剂，但在解释添加氨甲酰磷酸数小时后观察到的效应时必须谨慎，因为这些效应可能是由氰酸盐引起的。
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