Bogoliubova I O, Parfenov V N
Institute of Cytology RAS, St. Petersburg.
Tsitologiia. 2000;42(9):884-90.
The distribution of pre-mRNA splicing factors (snRNPs and SR-protein SC35) was studied in the nuclei of early and late 2-cell mouse embryos using light and electron immunocytochemistry. The splicing factors were shown to be present in the nuclei before the beginning of embryonic transcription. In inactive nuclei of early 2-cell embryos, SR-protein SC35 is concentrated in clusters of intranuclear granules to be absent from the karyoplasm. After the beginning of embryonic transcription activation, concentration of splicing factors in the karyoplasm increases. The data obtained are in accordance with the idea that some particular nuclear domains may be storage sites for pre-mRNA splicing factors for constant recruiting of splicing factors to perichromatin fibrils.
利用光镜和电镜免疫细胞化学技术，研究了小鼠2 -细胞早期和晚期胚胎细胞核中前体mRNA剪接因子（小核核糖核蛋白和SR蛋白SC35）的分布情况。结果表明，剪接因子在胚胎转录开始之前就已存在于细胞核中。在2 -细胞早期胚胎的静止细胞核中，SR蛋白SC35集中在内核颗粒簇中，核质中不存在。胚胎转录激活开始后，核质中剪接因子的浓度增加。所获得的数据符合这样一种观点，即某些特定的核区域可能是前体mRNA剪接因子的储存位点，以便将剪接因子持续招募到染色质周围纤维中。
