Herzog H
Department of Crop Science in Tropics and Subtropics, Humboldt University Berlin, Germany.
Isotopes Environ Health Stud. 2000;36(2):111-21. doi: 10.1080/10256010008032937.
Investigations were carried out on Vigna unguiculata L. Walp. to estimate the distribution and utilization of 15N in different organs after stem injection during vegetative, flowering and pod filling stage. During flowering effects of water deficit were also examined. In well watered plants, within 4 days after injection, 65% of 15N accumulated in leaves. This was drastically reduced to 42% by water deficit. 15N accumulation in stems increased under water deficit. The translocation of 15N from the stem base to roots were not altered by water deficit. During pod filling 62% of recovered 15N in the plants had accumulated in seeds, 24% in leaves and 11% in stems within 4 days, whereas the uptake of nitrogen in pod walls and roots remained low (2%). These results demonstrate that the method of injecting very small quantities (1 mg/plant) of 15N into the stem base allows an exact and detailed quantitative assessment of N translocation/distribution with regard to different organs, different growth stages and different treatments.
对豇豆进行了研究，以估计在营养期、开花期和结荚期茎部注射后15N在不同器官中的分布和利用情况。在开花期还研究了水分亏缺的影响。在水分充足的植株中，注射后4天内，65%的15N积累在叶片中。水分亏缺使这一比例急剧降至42%。水分亏缺时茎中15N的积累增加。水分亏缺并未改变15N从茎基部向根部的转运。在结荚期，4天内植株中回收的15N有62%积累在种子中，24%在叶片中，11%在茎中，而荚壁和根中氮的吸收量仍然很低（2%）。这些结果表明，向茎基部注射极少量（1毫克/株）15N的方法能够对不同器官、不同生长阶段和不同处理下氮的转运/分布进行准确而详细的定量评估。
