Hashimoto M, Hatanaka Y, Nabeta K
Department of Bioresource Science, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Bioorg Med Chem Lett. 2000 Nov 6;10(21):2481-3. doi: 10.1016/s0960-894x(00)00499-6.
4-[3-(Trifluoromethyl) diazirinyl] cinnamic acid derivatives were synthesized to elucidate properties of phenylalanine ammonia-lyase (PAL). 2-Methoxy and 2-biotinylated alkoxy compounds have inhibitory activity on the formation of phenylalanine from cinnamic acid. Specific photolabeling of the enzyme was detected using biotinylated derivatives without the use of radioisotopes. The results indicated that the 4-[3-(trifluoromethyl) diazirinyl] skeleton will be a suitable photoreactive compound to elucidate regulation of phenylpropanoid biosynthesis.
合成了4-[3-(三氟甲基)重氮丙啶基]肉桂酸衍生物以阐明苯丙氨酸解氨酶（PAL）的性质。2-甲氧基和2-生物素化的烷氧基化合物对肉桂酸形成苯丙氨酸具有抑制活性。使用生物素化衍生物检测到该酶的特异性光标记，而无需使用放射性同位素。结果表明，4-[3-(三氟甲基)重氮丙啶基]骨架将是阐明苯丙烷类生物合成调控的合适光反应性化合物。
