Richmond P, Borrow R, Goldblatt D, Findlow J, Martin S, Morris R, Cartwright K, Miller E
Immunisation Division, Communicable Disease Surveillance Centre, Public Health Laboratory Service, London, United Kingdom.
J Infect Dis. 2001 Jan 1;183(1):160-3. doi: 10.1086/317646. Epub 2000 Nov 14.
To test for immunologic memory after a single dose of meningococcal C conjugate (MCC) vaccine in toddlers, 226 children 12-18 months old were randomized to receive 1 of 3 MCC vaccines, with a C polysaccharide booster 6 months later. The protein conjugate was diphtheria mutant toxoid in 2 vaccines (MCC-CRM(197)) and was tetanus toxoid in the third (MCC-TT). One month after the MCC vaccines, 91%-100% of children had serum bactericidal antibody (SBA) titers > or =8, and 89%-100% had a > or =4-fold increase. Geometric mean titer (GMT) increased from <4 to 215 (95% confidence interval [CI], 166-279). MCC-TT induced higher SBA GMTs (P<.001) and higher proportions with SBA > or =8 (P=.02) than did the MCC-CRM(197) vaccines. By 6 months, GMTs had decreased to 55.1 (95% CI, 40-76), but IgG antibody avidity increased (P<.001). Induction of immunologic memory was confirmed by a GMT of 1977 (range, 1535-2547) after the polysaccharide booster and a further increase in avidity. This evidence justified the use of a single dose in a catch-up immunization program for children 1-18 years old.
为检测幼儿单剂量脑膜炎球菌C结合疫苗（MCC）接种后的免疫记忆，226名12至18个月大的儿童被随机分为3组，分别接种3种MCC疫苗中的1种，并在6个月后接种C多糖加强疫苗。2种疫苗（MCC-CRM(197)）中的蛋白结合物为白喉突变类毒素，第3种（MCC-TT）中的蛋白结合物为破伤风类毒素。接种MCC疫苗1个月后，91%-100%的儿童血清杀菌抗体（SBA）效价≥8，89%-100%的儿童SBA效价有≥4倍的增长。几何平均滴度（GMT）从<4增至215（95%置信区间[CI]，166-279）。与MCC-CRM(197)疫苗相比，MCC-TT诱导产生的SBA GMT更高（P<0.001），SBA≥8的儿童比例也更高（P=0.02）。到6个月时，GMT降至55.1（95%CI，40-76），但IgG抗体亲和力增加（P<0.001）。多糖加强疫苗接种后GMT为1977（范围，1535-2547），且亲和力进一步增加，从而证实了免疫记忆的诱导。这一证据证明在1至18岁儿童的补种免疫计划中使用单剂量疫苗是合理的。
