Jandik P, Cheng J, Jensen D, Manz S, Avdalovic N
Dionex Corporation, 1228 Titan Way, Sunnyvale, California 94088-3603, USA.
Anal Biochem. 2000 Dec 1;287(1):38-44. doi: 10.1006/abio.2000.4849.
A method is described for enhancing retention of arginine on a pellicular anion-exchange column. Arginine exhibits adjustable increases of retention time that are dependent on the acidity of standard or sample matrix. This effect is based on interactions of the protonated form of arginine with the residual cation-exchange groups on the core beads of pellicular particles. The relative magnitude of retention time shift of arginine is evaluated for identical concentrations of hydrochloric, sulfuric, and perchloric acids. Although the direct addition of acid is very effective in influencing the retention of arginine, it affects peak shapes and retention of other peaks in the chromatographic separation. The new technique-acid coinjection-achieves a similar retention enhancement for arginine with only a minimal effect on the rest of the separation. Detection limits, reproducibility results, and calibration data are presented for the chromatography of amino acids with acid coinjection. Improved resolution of arginine is demonstrated with chromatograms of soybean hydrolysate and cell culture samples.
描述了一种增强精氨酸在薄壳阴离子交换柱上保留的方法。精氨酸的保留时间呈现出可调节的增加，这取决于标准品或样品基质的酸度。这种效应基于精氨酸的质子化形式与薄壳颗粒核心珠上残留阳离子交换基团之间的相互作用。对于相同浓度的盐酸、硫酸和高氯酸，评估了精氨酸保留时间偏移的相对幅度。虽然直接添加酸对影响精氨酸的保留非常有效，但它会影响色谱分离中峰的形状和其他峰的保留。新技术——酸共注入——实现了对精氨酸类似的保留增强，而对分离的其他部分影响最小。给出了采用酸共注入进行氨基酸色谱分析的检测限、重现性结果和校准数据。大豆水解物和细胞培养样品的色谱图证明了精氨酸分辨率的提高。
