Jericho K W, Ho J, Kozub G C
Health Canada, Population and Public Health Branch, Animal Diseases Research Institute, Lethbridge, Alberta.
J Food Prot. 2000 Nov;63(11):1523-8. doi: 10.4315/0362-028x-63.11.1523.
The operation of the high-line speed cattle abattoir studied follows a plant-created hazard analysis and critical control point (HACCP) plan that is recognized by the Canadian Food Inspection Agency. Measurement of bioaerosols is not a part of this plan. In this study CFUs in air of selected abattoir processes were enumerated after impinging air onto tryptic soy agar plates with a slit air sampler for 10 to 20 min. The total viable count (TVC) per liter of air was calculated for each sample following incubation at 30 degrees C for 24 h. Monthly samples were collected on the hide removal floor and the carcass dressing floor from March 1998 to April 1999. Mud tag, dirt, and wetness of incoming hides were scored subjectively on the hide removal floor. The other processes were sampled in 3 separate months. The TVC at two locations on the hide removal floor (center of hide removal floor [CHF] and top of hide puller [THP]) had a strong association to each other (r = 0.84; P < 0.001). The mean TVC at the CHF and THP was 10.0 and 11.5, respectively, and the TVC for individual samples ranged from 2 to 42 at these locations. The TVC means for all the other processes ranged from 0.01 to 0.7. Tag and TVC on the hide removal floor had a different seasonal distribution with TVC being highest in the warm months (April to October 1998) and lowest for November to April 1999. No significant relations between TVC and the dirt and wetness variables were evident for the CHF and THP locations on the hide removal floor. It was concluded that the control of aerosols in the hide removal floor should be treated as a critical control point in the HACCP plan.
所研究的高线速肉牛屠宰场的运营遵循加拿大食品检验局认可的工厂制定的危害分析与关键控制点（HACCP）计划。生物气溶胶的测量并非该计划的一部分。在本研究中，使用狭缝空气采样器将空气冲击到胰蛋白胨大豆琼脂平板上10至20分钟后，对选定屠宰场工序空气中的菌落形成单位（CFU）进行计数。将每个样品在30摄氏度下培养24小时后，计算每升空气中的总活菌数（TVC）。1998年3月至1999年4月期间，每月在剥皮车间和胴体加工车间采集样品。在剥皮车间对进厂牛皮的泥污标签、污垢和湿度进行主观评分。其他工序在3个不同月份进行采样。剥皮车间两个位置（剥皮车间中心[CHF]和剥皮机顶部[THP]）的TVC彼此之间有很强的相关性（r = 0.84；P < 0.001）。CHF和THP处的平均TVC分别为10.0和11.5，这些位置单个样品的TVC范围为2至42。所有其他工序的TVC平均值在0.01至0.7之间。剥皮车间的标签和TVC有不同的季节分布，TVC在温暖月份（1998年4月至10月）最高，1999年11月至4月最低。在剥皮车间的CHF和THP位置，TVC与污垢和湿度变量之间没有明显的显著关系。得出的结论是，剥皮车间气溶胶的控制应被视为HACCP计划中的一个关键控制点。
