Nezlin L P, Schild D
Institute of Development Biology, Russian Academy of Sciences, Moscow.
Cell Tissue Res. 2000 Oct;302(1):21-9. doi: 10.1007/s004410000208.
The structure of the olfactory bulb in tadpoles of Xenopus laevis (stages 54-56) was studied using axon tracing (with biocytin or low-weight dextran) and immunocytochemical techniques. Filling the olfactory nerve with biocytin made the nerve layer and the glomeruli visible. Dye injections into the glomerular layer labeled the lateral olfactory tract. Vice versa, dye injections into the lateral olfactory tract made mitral cells and their glomerular branching patterns visible. Anti-GABA antiserum stained periglomerular and granule cells, while the olfactory nerve and mitral cells were labeled by antiglutamate antiserum. We describe the layering, the numbers of cells and glomeruli, and their localization in both the main and the accessory olfactory bulb.
利用轴突追踪技术（使用生物素或低分子量葡聚糖）和免疫细胞化学技术，对非洲爪蟾蝌蚪（54 - 56期）嗅球的结构进行了研究。用生物素填充嗅神经后，神经层和肾小球可见。向肾小球层注射染料标记了外侧嗅束。反之，向外侧嗅束注射染料则使二尖瓣细胞及其肾小球分支模式可见。抗GABA抗血清对球周细胞和颗粒细胞进行了染色，而嗅神经和二尖瓣细胞则被抗谷氨酸抗血清标记。我们描述了主嗅球和副嗅球的分层、细胞和肾小球的数量及其定位。
