Trieb K, Kohlbeck R, Lang S, Klinger H, Blahovec H, Kotz R
Department of Orthopedics, University of Vienna, Austria.
J Cancer Res Clin Oncol. 2000 Nov;126(11):667-70. doi: 10.1007/s004320000167.
Heat shock proteins (hsp) are involved in tumor immunity, and a correlation with survival, occurrence of metastases, and drug resistance has been reported. It was the aim of this study to investigate the expression of heat shock proteins in chondrosarcomas and chondromas.
Hsp expression was investigated immunohistochemically on paraffin-embedded sections of 37 consecutive patients (24 male and 13 female, mean age 48 years) with chondrosarcoma and of ten patients (six male, four female, mean age 36 years) with chondroma.
Chondromas showed a positive staining for hsp27 in 100%, for hsp60 in 30%, for hsp72 in 80%, for hsp73 in 80%, and for hsp90 in 90%. In chondrosarcoma a decreased expression was found for hsp27 (62% positive, P<0.05) and hsp72 (43% positive, P<0.05), whereas no significant difference to chondromas was detected in the expression of hsp60 (49% positive), hsp73 and hsp90 (73% and 81% positive, respectively). In addition, hsp72 expression showed a correlation with differentiation of the tumors (P<0.05); the lowest hsp72 expression was found in G3 chondrosarcomas (only 13% positive). No correlation with respect to differentiation was found for the expression of the other hsps.
This study shows a different expression of hsps in chondrosarcomas and chondromas. Together with the correlation of hsp72 expression with low differentiation, this finding could lead to new experimental and diagnostic strategies.
热休克蛋白（hsp）参与肿瘤免疫，且已有报道称其与生存率、转移发生及耐药性相关。本研究旨在调查热休克蛋白在软骨肉瘤和软骨瘤中的表达情况。
采用免疫组织化学方法，对37例连续的软骨肉瘤患者（24例男性，13例女性，平均年龄48岁）和10例软骨瘤患者（6例男性，4例女性，平均年龄36岁）的石蜡包埋切片进行hsp表达研究。
软骨瘤中hsp27的阳性染色率为100%，hsp60为30%，hsp72为80%，hsp73为80%，hsp90为90%。在软骨肉瘤中，hsp27（阳性率62%，P<0.05）和hsp72（阳性率43%，P<0.05）的表达降低，而hsp60（阳性率49%）、hsp73和hsp90（分别为阳性率73%和81%）的表达与软骨瘤相比无显著差异。此外，hsp72表达与肿瘤分化相关（P<0.05）；在G3级软骨肉瘤中hsp72表达最低（仅13%阳性）。其他hsp的表达与分化无相关性。
本研究显示软骨肉瘤和软骨瘤中hsp的表达不同。结合hsp72表达与低分化的相关性，这一发现可能会带来新的实验和诊断策略。
