来自大肠杆菌K-12 F性因子的DNA分子克隆。
Molecular cloning of DNA from F sex factor of Escherichia coli K-12.
作者信息
Skurray R A, Nagaishi H, Clark A J
出版信息
Proc Natl Acad Sci U S A. 1976 Jan;73(1):64-8. doi: 10.1073/pnas.73.1.64.
We describe the molecular cloning of various DNA segments generated by partial EcoRI endonuclease digestion of the sex factor F. These segments have been analyzed by agarose gel electrophoresis of EcoRI digests and were arranged in a series of overlapping fragments using the EcoRI fragment map of F established by H. Ohtsubo and E. Ohtsubo. The clones isolated demonstrate one or more of the following F-specified functions: inhibition of female-specific phage (T7) multiplication, formation of F pili, surface exclusion, or immunity to lethal zygosis. These properties are discussed in terms of the EcoRI fragments of F that specify them.
我们描述了通过性因子F的部分EcoRI核酸内切酶消化产生的各种DNA片段的分子克隆。这些片段已通过EcoRI消化产物的琼脂糖凝胶电泳进行分析，并利用大坪浩和大坪悦子建立的F的EcoRI片段图谱排列成一系列重叠片段。分离得到的克隆显示出以下一种或多种由F指定的功能：抑制雌性特异性噬菌体（T7）增殖、形成F菌毛、表面排斥或对致死性合子形成免疫。根据指定这些特性的F的EcoRI片段对这些特性进行了讨论。
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