Oda Y, Ito M
Upland Agriculture Research Center, Hokkaido National Agricultural Experiment Station, Memuro, Kasai, Hokkaido, 082-0071, Japan.
Curr Microbiol. 2000 Dec;41(6):392-5. doi: 10.1007/s002840010156.
A strain YF43, which can grow on sucrose as rapidly as glucose, was isolated by mutation from Lactobacillus amylovorus JCM 1126, the type strain defective in sucrose utilization. Exogenous sucrose stimulated the production of invertase by strains YF43 and JCM 1126 simultaneously. In a medium containing fructooligosaccharide as the sole carbon source, the cells of strain YF43 showed high invertase activity in spite of poor growth. The two invertases produced in the cells grown on sucrose and fructooligosaccharide were an identical beta-fructofuranosidase, as judged from properties of partially purified enzymes. These observations indicated that strain YF43 is a mutant improved for permeation of sucrose and not derepressed for the synthesis of invertase.
从不能利用蔗糖的模式菌株解淀粉乳杆菌JCM 1126经诱变分离出了一株YF43菌株，该菌株利用蔗糖的速度与利用葡萄糖的速度一样快。外源蔗糖同时刺激了YF43菌株和JCM 1126菌株产生转化酶。在以低聚果糖作为唯一碳源的培养基中，尽管YF43菌株生长不佳，但其细胞仍表现出较高的转化酶活性。根据部分纯化酶的特性判断，在蔗糖和低聚果糖上生长的细胞中产生的两种转化酶是同一种β-呋喃果糖苷酶。这些观察结果表明，YF43菌株是一株蔗糖渗透能力得到改善的突变体，而非转化酶合成去阻遏的突变体。
