Inami T, Obara T, Moriyama M, Arakawa Y, Abe K
Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan.
Virology. 2000 Nov 25;277(2):330-5. doi: 10.1006/viro.2000.0621.
Recently, we identified TT virus (TTV) isolates from nonhuman primates and named them simian TTV (s-TTV). To characterize the genomic structure of these isolates in more detail, the full-length nucleotide sequence of the s-TTV isolate (designated s-TTV CH65-1), recovered from a chimpanzee born in West Africa, was amplified by nested PCR with inverted primers deduced from the untranslated region of s-TTV DNA. CH65-1 was composed of 3899 nucleotides (nt) and had two open reading frames (ORF) spanning 2295 nt (ORF1) and 402 nt (ORF2). The sequence had only 52.3% similarity to the prototype TA278 human isolate. Phylogenetic analysis demonstrated that CH65-1 was distinct from the human TTV isolates. These results suggested that s-TTV may represent a new TTV-like viral species or genus.
最近，我们从非人类灵长类动物中鉴定出了TT病毒（TTV）分离株，并将它们命名为猿猴TTV（s-TTV）。为了更详细地描述这些分离株的基因组结构，我们使用从s-TTV DNA非翻译区推导的反向引物，通过巢式PCR扩增了从一只出生于西非的黑猩猩身上获得的s-TTV分离株（命名为s-TTV CH65-1）的全长核苷酸序列。CH65-1由3899个核苷酸（nt）组成，有两个开放阅读框（ORF），分别跨越2295 nt（ORF1）和402 nt（ORF2）。该序列与原型TA278人类分离株的相似性仅为52.3%。系统发育分析表明，CH65-1与人类TTV分离株不同。这些结果表明，s-TTV可能代表一种新的TTV样病毒种或属。
