前沿探索：变构核酶的创制
Working at the cutting edge: the creation of allosteric ribozymes.
作者信息
Warashina M, Kuwabara T, Taira K
机构信息
National Institute for Advanced Interdisciplinary Research AIST, MITI 305-8562, Tsukuba Science City, Japan.
出版信息
Structure. 2000 Nov 15;8(11):R207-12. doi: 10.1016/s0969-2126(00)00517-7.
Abstract
The appropriate folding of catalytic RNA is a prerequisite for effective catalysis. A novel ribozyme, the maxizyme, has been generated and its activity can be controlled allosterically. The maxizymes work both in vitro and in vivo indicating the potential utility of this novel class of ribozyme as a gene-inactivating agent with a biosensor function.
摘要
催化性RNA的正确折叠是有效催化的前提条件。一种新型核酶——最大酶已被研制出来，其活性可通过别构作用进行控制。最大酶在体外和体内均能发挥作用，这表明这类新型核酶作为一种具有生物传感器功能的基因失活剂具有潜在的应用价值。
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引用本文的文献
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Significantly higher activity of a cytoplasmic hammerhead ribozyme than a corresponding nuclear counterpart: engineered tRNAs with an extended 3' end can be exported efficiently and specifically to the cytoplasm in mammalian cells.胞质锤头状核酶的活性显著高于相应的核内对应物：具有延长3'末端的工程化tRNA能够在哺乳动物细胞中高效且特异地输出至胞质。
Nucleic Acids Res. 2001 Jul 1;29(13):2780-8. doi: 10.1093/nar/29.13.2780.
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