Yamada K, Elliott W M, Hayashi S, Brattsand R, Roberts C, Vitalis T Z, Hogg J C
University of British Columbia Pulmonary Research Laboratory, St Paul's Hospital, Vancouver, British Columbia, Canada.
J Allergy Clin Immunol. 2000 Nov;106(5):844-51. doi: 10.1067/mai.2000.110473.
Steroid-resistant asthma develops after adenoviral bronchiolitis.
We sought to determine the effect of steroids on allergic lung inflammation in the presence of latent adenoviral infection.
Guinea pigs with latent adenoviral (n = 12) or sham (n = 12) infections were sensitized and challenged with ovalbumin (OA) or sham sensitized and challenged with saline solution. The effect of steroids (20 mg/kg administered intraperitoneally) on OA-induced lung inflammation was examined by using quantitative histology as the outcome measure.
Latent adenoviral infection increased CD8(+) cells in the airway wall and CD8(+) cells, macrophages, B cells, and CD4(+) cells in the lung parenchyma. Ovalbumin challenge, on the other hand, increased eosinophils, macrophages, B cells, and CD4(+) cells in both the airway wall and lung parenchyma independent of the effect of latent adenoviral infection. In the sham-infected groups steroid treatment caused the expected reduction in the eosinophilic infiltrate induced by OA challenge in the airways without affecting the other cells. In the presence of both latent adenoviral infection and OA challenge, steroid treatment had no effect on allergen-induced eosinophilia but reduced CD8(+) cells in the airways and CD8(+) cells, CD4(+) cells, and B cells in the parenchyma.
Latent adenoviral infection and OA challenge result in different types of lung inflammation, and the presence of latent adenoviral infection causes OA-induced eosinophilic airway inflammation to become steroid resistant.
类固醇抵抗性哮喘在腺病毒性细支气管炎后发生。
我们试图确定在潜伏性腺病毒感染存在的情况下类固醇对过敏性肺部炎症的影响。
将患有潜伏性腺病毒感染（n = 12）或假感染（n = 12）的豚鼠用卵清蛋白（OA）致敏并激发，或假致敏并用盐溶液激发。以定量组织学作为结果指标，研究类固醇（20 mg/kg腹腔注射）对OA诱导的肺部炎症的影响。
潜伏性腺病毒感染增加了气道壁中的CD8(+)细胞以及肺实质中的CD8(+)细胞、巨噬细胞、B细胞和CD4(+)细胞。另一方面，卵清蛋白激发增加了气道壁和肺实质中的嗜酸性粒细胞、巨噬细胞、B细胞和CD4(+)细胞，这与潜伏性腺病毒感染的影响无关。在假感染组中，类固醇治疗使气道中由OA激发诱导的嗜酸性粒细胞浸润按预期减少，而不影响其他细胞。在潜伏性腺病毒感染和OA激发同时存在的情况下，类固醇治疗对变应原诱导的嗜酸性粒细胞增多无影响，但减少了气道中的CD8(+)细胞以及实质中的CD8(+)细胞、CD4(+)细胞和B细胞。
潜伏性腺病毒感染和OA激发导致不同类型的肺部炎症，并且潜伏性腺病毒感染的存在使OA诱导的嗜酸性气道炎症变得对类固醇耐药。
