Santos C P, Lanfredi R M
Instituto de Ciências Biológicas e Ambientais, Universidade Santa Ursula, 22231-040 Rio de Janeiro, RJ, Brasil.
Mem Inst Oswaldo Cruz. 2000 Nov-Dec;95(6):899-904. doi: 10.1590/s0074-02762000000600028.
The tegument of the polyopisthocotylean monogenean Atriaster heterodus Lebedev & Parukhin, 1969 was studied using transmission electron microscopy. The outer syncytial layer of the tegument is connected to the internal cell bodies by cytoplasmic extensions which interweave between the muscular fibres. The free surface of the syncytium has projections of the external membrane which are similar to microvilli. The undulating basal membrane, with numerous narrow elongate projections, is associated with the basal lamina situated between the syncytial and muscular layers. The cell bodies and syncytial layer of the tegument exhibit two types of vesicles, one with fibrous contents and one with electron-dense contents; these were analysed using two cytochemical tests, the E-PTA and alcian blue methods, used for the first time on monogeneans.
利用透射电子显微镜对1969年勒别杰夫和帕鲁欣发现的多后盘吸虫单殖吸虫异形异星吸虫的皮层进行了研究。皮层的外部合胞体层通过在肌纤维间交织的细胞质延伸与内部细胞体相连。合胞体的自由表面有类似于微绒毛的外膜突起。起伏的基膜有许多狭窄的细长突起,与位于合胞体层和肌肉层之间的基膜相关联。皮层的细胞体和合胞体层有两种类型的小泡,一种含有纤维状内容物,另一种含有电子致密内容物;使用两种细胞化学测试方法,即E-PTA和阿尔辛蓝方法对其进行了分析,这两种方法首次用于单殖吸虫。