Yoshida M, Suzuki T
Department of Cellular and Molecular Biology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
AIDS Res Hum Retroviruses. 2000 Nov 1;16(16):1639-45. doi: 10.1089/08892220050193083.
HTLV-1 Tax oncoprotein exerts pleiotropic effects on cellular regulatory systems, trans-activation and trans-repression of transcription and promotion of the cell cycle, through interaction with various cellular factors. Here, we report Tax-mediated inhibition of DNA topoisomerase I. The interaction of Tax with topoisomerase I was demonstrated in vitro and also in vivo, and inhibition of DNA relaxation activity of topoisomerase I was clearly shown. These results suggested that Tax has a novel potential to affect various cellular processes, such as transcription and maintenance of genomic stability, in which DNA topoisomerase I is involved.
人嗜T淋巴细胞病毒1型（HTLV-1）的Tax癌蛋白通过与多种细胞因子相互作用，对细胞调节系统、转录的反式激活和反式抑制以及细胞周期的促进发挥多效性作用。在此，我们报道Tax介导的对DNA拓扑异构酶I的抑制作用。Tax与拓扑异构酶I的相互作用在体外和体内均得到证实，并且拓扑异构酶I的DNA松弛活性受到明显抑制。这些结果表明，Tax具有影响各种细胞过程的新潜能，如DNA拓扑异构酶I所涉及的转录和基因组稳定性维持。
