Sumner C, Sabot A, Turner K, Krause S
Department of Chemistry, University of Sheffield, U.K.
Anal Chem. 2000 Nov 1;72(21):5225-32. doi: 10.1021/ac000411y.
A novel transducer based on the dissolution of biodegradable polymer films as a direct result of enzymatic reaction has been developed. Three polymers were investigated for use in the transducer: a poly(ester amide), which is degraded by the proteolytic enzyme alpha-chymotrypsin; a dextran hydrogel, which is degraded by dextranase; and poly(trimethylene) succinate, which is degraded by a lipase. Degradation of the polymer films was monitored by surface plasmon resonance (SPR) and impedance measurements. SPR was shown to be suitable for a greater variety of materials, since it does not require the polymer film to be electrically insulating. Rate of degradation was shown to be directly related to enzyme concentration for each polymer/enzyme couple. The poly(ester amide)/alpha-chymotrypsin couple proved to be the most sensitive. Degradation of the films was complete in less than 20 min for enzyme concentrations greater than 9 x 10(-9) mol dm-3. Enzyme concentrations as low as 4 x 10(-11) mol dm-3 were detected in less than 30 min. The transducer has great potential for the detection of enzyme concentrations as well as for use in immunosensing where the enzyme degrading the polymer would be the enzyme label.
一种基于酶促反应直接导致可生物降解聚合物薄膜溶解的新型传感器已被开发出来。研究了三种聚合物用于该传感器：一种聚（酯酰胺），可被蛋白水解酶α-胰凝乳蛋白酶降解；一种葡聚糖水凝胶，可被葡聚糖酶降解；以及聚（三亚甲基）琥珀酸酯，可被脂肪酶降解。通过表面等离子体共振（SPR）和阻抗测量来监测聚合物薄膜的降解情况。结果表明SPR适用于更多种类的材料，因为它不要求聚合物薄膜具有电绝缘性。对于每一对聚合物/酶，降解速率都与酶浓度直接相关。聚（酯酰胺）/α-胰凝乳蛋白酶这一对被证明是最敏感的。对于浓度大于9×10⁻⁹ mol dm⁻³的酶，薄膜在不到20分钟内就完全降解。在不到30分钟内就能检测到低至4×10⁻¹¹ mol dm⁻³的酶浓度。该传感器在检测酶浓度以及用于免疫传感方面具有巨大潜力，在免疫传感中，降解聚合物的酶将作为酶标记物。
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