Laiko V V, Moyer S C, Cotter R J
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Anal Chem. 2000 Nov 1;72(21):5239-43. doi: 10.1021/ac000530d.
A new sample ionization technique, atmospheric pressure matrix-assisted laser desorption/ionization (AP MALDI), was coupled with a commercial ion trap mass spectrometer. This configuration enables the application-specific selection of external atmospheric ionization sources: the electrospray/APCI (commercially available) and AP MALDI (built in-house), which can be readily interchanged within minutes. The detection limit of the novel AP MALDI/ion trap is 10-50 fmol of analyte deposited on the target surface for a four-component mixture of peptides with 800-1700 molecular weight. The possibility of peptide structural analysis by MS/MS and MS3 experiments for AP MALDI-generated ions was demonstrated for the first time.
一种新的样品电离技术——大气压基质辅助激光解吸/电离（AP MALDI），与一台商用离子阱质谱仪联用。这种配置能够根据应用需求选择外部大气压电离源：电喷雾/大气压化学电离（市售）和AP MALDI（自制），它们可在数分钟内轻松互换。对于分子量为800 - 1700的四组分肽混合物，新型AP MALDI/离子阱的检测限为沉积在靶表面的10 - 50飞摩尔分析物。首次证明了通过MS/MS和MS3实验对AP MALDI产生的离子进行肽结构分析的可能性。
