El-Husseini A E, Schnell E, Chetkovich D M, Nicoll R A, Bredt D S
Department of Physiology, University of California, San Francisco 94143, USA.
Science. 2000 Nov 17;290(5495):1364-8.
PSD-95 is a neuronal PDZ protein that associates with receptors and cytoskeletal elements at synapses, but whose function is uncertain. We found that overexpression of PSD-95 in hippocampal neurons can drive maturation of glutamatergic synapses. PSD-95 expression enhanced postsynaptic clustering and activity of glutamate receptors. Postsynaptic expression of PSD-95 also enhanced maturation of the presynaptic terminal. These effects required synaptic clustering of PSD-95 but did not rely on its guanylate kinase domain. PSD-95 expression also increased the number and size of dendritic spines. These results demonstrate that PSD-95 can orchestrate synaptic development and are suggestive of roles for PSD-95 in synapse stabilization and plasticity.
PSD - 95是一种神经元PDZ蛋白，它在突触处与受体和细胞骨架成分相关联，但其功能尚不确定。我们发现，海马神经元中PSD - 95的过表达可驱动谷氨酸能突触的成熟。PSD - 95的表达增强了突触后谷氨酸受体的聚集和活性。PSD - 95在突触后的表达也增强了突触前终末的成熟。这些效应需要PSD - 95的突触聚集，但不依赖于其鸟苷酸激酶结构域。PSD - 95的表达还增加了树突棘的数量和大小。这些结果表明，PSD - 95可以协调突触发育，并提示其在突触稳定和可塑性方面的作用。
