Moossa A R, Zarins C K, Skinner D B
Surgery. 1976 Jan;79(1):60-4.
Twenty-seven anesthetized dogs were surface cooled at 4 to 6 degrees C. or 15 to 20 degrees C. Circulation was provided by the mechanical ventricular assist in 23 dogs. After 24 or 48 hours of in situ preservation, the kidneys were transplanted into the necks or iliac fossas of anephric recipients. Renal function was preserved in the cadaver for up to 48 hours at 15 to 20 degrees C. by maintaining a pulsatile circulation. Further cooling to 4 to 6 degrees C. caused progressive deterioration in renal function. The nonperfused kidneys kept in situ at 4 to 6 degrees C. did not produce any urine after transplantation. If the practical problems of total body cooling are solved, in situ preservation of multiple organs in the cadaver would increase the number of available organs for transplantation.
27只麻醉犬分别在4至6摄氏度或15至20摄氏度下进行体表降温。23只犬通过机械心室辅助维持循环。在原位保存24或48小时后，将肾脏移植到无肾受体的颈部或髂窝。通过维持搏动性循环，在15至20摄氏度下，尸体肾脏的功能可保存长达48小时。进一步降温至4至6摄氏度会导致肾功能逐渐恶化。在4至6摄氏度下原位保存的未灌注肾脏移植后不产生任何尿液。如果解决全身降温的实际问题，尸体中多个器官的原位保存将增加可用于移植的器官数量。
