Cianciulli A M, Morace E, Coletta A M, Occhipinti E, Gandolfo G M, Leonardo G, Carapella C M
Department of Clinical Pathology, Regina Elena Cancer Institute, Rome, Italy.
J Neurooncol. 2000 Jun;48(2):95-101. doi: 10.1023/a:1006420921159.
Models describing progression in the genetic derangement of glial tumors have shown chromosomal loss and gain occurring most frequently in high-grade lesions, suggesting that identification of these aberrations may be prognostically significant. In this study, Fluorescence in situ hybridization (FISH) has been used to determine, and to confirm, loss and gain of chromosomes 1, 8, 10, 12 and 17, in formalin-fixed, paraffin-embedded brain biopsy tissue taken from 60 brain gliomas submitted to surgical resection or stereotactic biopsy. FISH analysis may be a valuable adjunct to histological grading. The results showed that this molecular cytogenetic technique is an important clinical and experimental tool that provides new insight on genetic alterations, confirming gain and loss of genetic material that occurs at the initiation and progression of human glioma. Our data suggests that potentially useful prognostic information may be obtained through this approach. Monosomy 10 was the most statistically significant negative predictor of patient survival, showing a significant correlation with the histological grading.
描述胶质细胞瘤基因紊乱进展的模型显示，染色体缺失和增加在高级别病变中最为常见，这表明识别这些畸变可能具有预后意义。在本研究中，荧光原位杂交（FISH）已被用于确定并确认取自60例接受手术切除或立体定向活检的脑胶质瘤的福尔马林固定、石蜡包埋脑活检组织中1、8、10、12和17号染色体的缺失和增加。FISH分析可能是组织学分级的一个有价值的辅助手段。结果表明，这种分子细胞遗传学技术是一种重要的临床和实验工具，它为基因改变提供了新的见解，证实了人类胶质瘤发生和进展过程中遗传物质的增加和缺失。我们的数据表明，通过这种方法可能获得潜在有用的预后信息。10号染色体单体是患者生存最具统计学意义的负性预测指标，与组织学分级显著相关。
