Perry A, Tonk V, McIntire D D, White C L
Department of Pathology, The University of Texas Southwestern Medical School, Dallas 75235-9072, USA.
Am J Clin Pathol. 1997 Aug;108(2):166-74. doi: 10.1093/ajcp/108.2.166.
Astrocytomas contain nonrandom chromosomal abnormalities that recently have been correlated with shortened patient survival. Two frequently reported aberrations are trisomy 7 and monosomy 10. We assessed the numerical complement of chromosomes 7 and 10 in formalin-fixed, paraffin-embedded brain biopsy tissue from 28 diffuse astrocytomas by in situ hybridization using a nonfluorescent enzymatic detection system. Clinical follow-up of at least 5 years was available in 26 cases (93%). Monosomy 10 was identified in 7 cases (25%): astrocytoma, 1 case; anaplastic astrocytoma, 1 case; and glioblastoma, 5 cases. Trisomy 7 was identified in 11 cases (39%): astrocytoma, 5 cases; glioblastoma, 6 cases. Multivariate analysis revealed that monosomy 10 was the most statistically significant negative predictor of patient survival. Numerical chromosomal abnormalities are detectable in astrocytomas in archival tissue using interphase cytogenetics and nonfluorescent light microscopy. Although larger studies are required, our data suggest that potentially useful prognostic information may be obtained with this approach.
星形细胞瘤存在非随机的染色体异常，最近的研究表明这些异常与患者生存期缩短相关。两种常见的畸变是7号染色体三体和10号染色体单体。我们使用非荧光酶检测系统，通过原位杂交技术评估了28例弥漫性星形细胞瘤的福尔马林固定、石蜡包埋脑活检组织中7号和10号染色体的数目。26例（93%）患者有至少5年的临床随访资料。10号染色体单体在7例（25%）中被检测到：星形细胞瘤1例；间变性星形细胞瘤1例；胶质母细胞瘤5例。7号染色体三体在11例（39%）中被检测到：星形细胞瘤5例；胶质母细胞瘤6例。多因素分析显示，10号染色体单体是患者生存期最具统计学意义的负性预测指标。使用间期细胞遗传学和非荧光光学显微镜可在存档组织的星形细胞瘤中检测到染色体数目异常。尽管需要更大规模的研究，但我们的数据表明，通过这种方法可能获得有用的预后信息。
