Centre de Transfusió i Banc de Teixits. Tarragona.
An Esp Pediatr. 2000 Sep;53(3):249-52.
ABO-hemolytic disease occurs when ABO-incompatibility is present between the mother and the fetus. The objective of the present study was to analyze ABO-hemolytic disease in our center, to compare two serologic tests to diagnose it and to evaluate hematocrit values in cord blood samples.
ABO and Rh(D) group was found in 881 blood samples. Umbilical cord blood samples were obtained and direct antiglobulin test (DAT), eluate and microhematocrit were performed.
ABO-incompatibility was found in 134 (15.3%) cases. DAT was positive in 31.6% of patients studied with ABO-incompatibility and the eluate identified the antibody in 68.4% of studied patients (p = 0.001). Hematocrit values were lower in the group with ABO-incompatibility than in the ABO-compatible group (44 +/- 7.8% vs. 46.5 +/- 6.5%; p = 0.029).
Although severe ABO hemolytic disease is rare, ABO incompatibility is detected with current serologic tests and it causes a certain degree of hemolysis in newborn infants.
当母亲与胎儿之间存在ABO血型不相容时，会发生ABO溶血病。本研究的目的是分析我院的ABO溶血病情况，比较两种诊断该病的血清学检测方法，并评估脐血样本中的血细胞比容值。
对881份血样进行ABO和Rh（D）血型鉴定。采集脐血样本，进行直接抗球蛋白试验（DAT）、洗脱液检测和微量血细胞比容测定。
发现134例（15.3%）存在ABO血型不相容。在进行ABO血型不相容检测的患者中，31.6%的患者DAT呈阳性，洗脱液在68.4%的受检患者中鉴定出抗体（p = 0.001）。ABO血型不相容组的血细胞比容值低于ABO血型相容组（44±7.8%对46.5±6.5%；p = 0.029）。
虽然严重的ABO溶血病很少见，但目前的血清学检测可检测到ABO血型不相容，且其会导致新生儿一定程度的溶血。
