Segura F, Brioso M A, Gómez J F, Machado J D, Borges R
Unidad de Farmacología, Facultad de Medicina, Universidad de La Laguna, Tenerife, Spain.
J Neurosci Methods. 2000 Nov 30;103(2):151-6. doi: 10.1016/s0165-0270(00)00309-5.
Amperometry is a widely used technique for monitoring the secretion of catecholamines (CA) by exocytosis. The use of carbon fibre microelectrodes allows the on-line recording of CA released from a single secretory vesicle. Amperometric signals are generated by oxidation of the quantally released CA close to the electrode tip. Each event of exocytosis is called a secretory spike. Here we describe a program written for IGOR (Wavemetrics, Lake Oswego, OR, USA), which may be used to analyze amperometric signals off-line. The procedures allow, (i) digital filtering and analysis of the current noise, spike identification and calculation of spike kinetic parameters; (ii) spike review; (iii) pooling spikes and data to create galleries, tables and histograms of measured parameters which can be exported to a graphic format or files for further analysis.
安培测量法是一种广泛用于监测儿茶酚胺（CA）通过胞吐作用分泌的技术。碳纤维微电极的使用使得能够在线记录从单个分泌囊泡释放的CA。安培信号是由靠近电极尖端的定量释放的CA氧化产生的。每次胞吐事件都称为一个分泌峰。在这里，我们描述了一个为IGOR（Wavemetrics，美国俄勒冈州奥斯威戈湖）编写的程序，该程序可用于离线分析安培信号。这些程序允许：（i）对电流噪声进行数字滤波和分析、峰识别以及峰动力学参数的计算；（ii）峰回顾；（iii）汇总峰和数据以创建测量参数的图库、表格和直方图，这些可以导出为图形格式或文件以供进一步分析。
