用于生产L-苹果酸的固定化富马酸酶酶膜反应器的研究。
Study of an enzyme membrane reactor with immobilized fumarase for production of L-malic acid.
作者信息
Giorno L, Drioli E, Carvoli G, Cassano A, Donato L
机构信息
Research Institute on Membranes and Modelling of Chemical Reactors, IRMERC-CNR, University of Calabria, Via P. Bucci 17/C, 87030 Rende, CS, Italy.
出版信息
Biotechnol Bioeng. 2001 Jan 5;72(1):77-84. doi: 10.1002/1097-0290(20010105)72:1<77::aid-bit11>3.0.co;2-l.
The conversion of fumaric acid into L-malic acid by fumarase immobilized in a membrane reactor was analyzed experimentally. The enzyme was entrapped in asymmetric capillary membranes made of polysulfone. The performance of the reactor was evaluated in terms of conversion degree, reaction rate, and stability. The influence of operating conditions, such as amount of immobilized enzyme, substrate concentration, residence time, and axial flow rate, were investigated. The kinetic parameters K(m), V(max), and k(+2) were also measured. The stability of the immobilized enzyme was very good, showing no activity decay during more than 2 weeks of continuous operation.
对固定在膜反应器中的富马酸酶将富马酸转化为L - 苹果酸的过程进行了实验分析。该酶被包埋在由聚砜制成的不对称毛细管膜中。根据转化率、反应速率和稳定性对反应器的性能进行了评估。研究了操作条件的影响，如固定化酶的量、底物浓度、停留时间和轴向流速。还测量了动力学参数K(m)、V(max)和k(+2)。固定化酶的稳定性非常好，在连续运行超过2周的时间内没有活性衰减。
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