鼻咽癌中嗜银性核仁组成区（AgNORs）的形态计量分析
Morphometric analysis of argyrophilic nucleolar organizer regions (AgNORs) in nasopharyngeal carcinoma.
作者信息
Pak M W, To K F, Chen M H, Lo S Y, Lam P K, van Hasselt C A
机构信息
Division of Otorhinolaryngology, Department of Surgery, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong.
出版信息
Head Neck. 2000 Dec;22(8):760-4. doi: 10.1002/1097-0347(200012)22:8<760::aid-hed3>3.0.co;2-p.
BACKGROUND
Nucleolar organizer regions (NORs), which are loops of DNA containing ribosomal RNA genes, have been shown to correlate with cell proliferation and malignant transformation. Conventional eyeball measurement of silver staining NORs (AgNORs) is time-consuming and subject to error. The diagnostic value of AgNOR area in nasopharyngeal carcinoma (NPC) by computer-assisted morphometric analysis is evaluated.
METHODS
Silver-staining of NORs was applied to 23 paraffin sections of NPC containing both normal squamous epithelial and malignant cells. Various parameters of the AgNORs of these two cell types were analyzed by a computer-assisted image analysis system and then compared.
RESULTS
The mean AgNOR area, AgNOR/nuclear area ratio, and AgNOR area/count ratio of malignant tumors were statistically significantly higher than those for the normal epithelium. There was no significant difference in the AgNOR counts between the two cell types.
CONCLUSIONS
Computer-assisted morphometric analysis of AgNOR is an objective and reliable assessment method applicable to paraffin sections of NPC. The AgNOR area and its derivatives may aid in the diagnosis of NPC.
背景
核仁组织区（NORs）是包含核糖体RNA基因的DNA环，已被证明与细胞增殖和恶性转化相关。传统的银染核仁组织区（AgNORs）眼球测量法耗时且容易出错。通过计算机辅助形态计量分析评估AgNOR面积在鼻咽癌（NPC）中的诊断价值。
方法
对23例包含正常鳞状上皮细胞和恶性细胞的鼻咽癌石蜡切片进行NORs银染。通过计算机辅助图像分析系统分析这两种细胞类型的AgNORs的各种参数，然后进行比较。
结果
恶性肿瘤的平均AgNOR面积、AgNOR/核面积比和AgNOR面积/计数比在统计学上显著高于正常上皮细胞。两种细胞类型之间的AgNOR计数没有显著差异。
结论
计算机辅助的AgNOR形态计量分析是一种适用于鼻咽癌石蜡切片的客观可靠的评估方法。AgNOR面积及其衍生指标可能有助于鼻咽癌的诊断。
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