类风湿关节炎中的胶原蛋白抗体和胶原蛋白-抗胶原蛋白免疫复合物

Collagen antibodies and collagen- anticollagen immune complexes in rheumatoid arthritis.

作者信息

Steffen C, Ludwig H, Knapp W, Thumb N, Eberl R, Frank O, Freilinger H

出版信息

Z Rheumatol. 1975 Nov-Dec;34(11-12):391-9.

Abstract

Serum, synovial fluid and synovial fluid cells of 14 patients with classic rheumatoid arthritis and of 5 controls were investigated serologically in regard to rheumatoid factors and collagen antibodies and by immunofluorescence double staining in regard to localization of collagen and gammaglobulin in fluid cells. Three patterns of staining were observed: a) gammaglobulin inclusions, b) gammaglobulin and collagen in identical localization, c) collagen inclusions. While distinct staining for gammaglobulin appeared only in seropositive cases, collagen inclusions were only observed in RA, however in seropositive and seronegative cases. In 5 RA cases collagen and gammaglobulin were demonstrated additionally in such identical localization that conclusion in regard to collagen-anticollagen immune complexes seems justified. 9 of 14 RA cases had collagen antibodies, all cases were collagen antibody positive which had collagen and gammaglobulin in identical localization in same inclusions.

摘要

对14例典型类风湿性关节炎患者及5例对照者的血清、滑液及滑液细胞进行了血清学研究，检测类风湿因子和胶原抗体，并通过免疫荧光双重染色观察胶原和γ球蛋白在滑液细胞中的定位。观察到三种染色模式：a）γ球蛋白包涵体；b）γ球蛋白和胶原位于相同定位；c）胶原包涵体。γ球蛋白的明显染色仅出现在血清阳性病例中，而胶原包涵体仅在类风湿性关节炎患者中观察到，血清阳性和血清阴性病例均有。在5例类风湿性关节炎病例中，还发现胶原和γ球蛋白位于相同定位，因此关于胶原-抗胶原免疫复合物的结论似乎是合理的。14例类风湿性关节炎病例中有9例有胶原抗体，所有在相同包涵体中胶原和γ球蛋白位于相同定位的病例胶原抗体均为阳性。