Wang A Y, Bobryshev Y V, Liang H, Cherian S M, Inder S J, Ashwell K W, Farnsworth A E, Lord R S
Surgical Professorial Unit, St. Vincent's Hospital, Darlinghurst, NSW, Australia.
J Submicrosc Cytol Pathol. 2000 Apr;32(2):209-19.
Aortocoronary saphenous vein bypass grafts undergo structural alterations within the arterialized vein, resulting in graft stenosis and failure. Areas of the acellular intima contribute to fissuring, cracking and ulceration, while areas of the media become highly vascular but thinned. This study aimed to examine the ultrastructural features of cell death, including apoptosis and necrosis, in non-atherosclerotic areas of the stenotic aortocoronary saphenous vein bypass grafts. Thirteen stenotic vein grafts were obtained at redo coronary artery bypass grafting. The ultrastructural features of cell death were analysed by electron microscopy. Typical features of necrosis, including focal areas of cytoplasmic oedema, plasmalemmal destruction and nuclear condensation with cytoplasmic organelle destruction, were observed throughout the intima and media. Features of apoptosis, including the presence of apoptotic bodies, were also identified in the hyperplastic intima and its adjacent media. Our observations suggest that both apoptosis and necrosis occur in non-atherosclerotic areas of stenotic aortocoronary saphenous vein bypass grafts.
主动脉冠状动脉大隐静脉旁路移植血管在动脉化静脉内会发生结构改变，导致移植血管狭窄和功能衰竭。无细胞内膜区域会出现裂隙、破裂和溃疡，而中膜区域血管高度增生但变薄。本研究旨在检查狭窄的主动脉冠状动脉大隐静脉旁路移植血管非动脉粥样硬化区域细胞死亡的超微结构特征，包括凋亡和坏死。在再次冠状动脉旁路移植术中获取了13条狭窄的静脉移植血管。通过电子显微镜分析细胞死亡的超微结构特征。在内膜和中膜均观察到坏死的典型特征，包括局部细胞质水肿、质膜破坏以及伴有细胞质细胞器破坏的核浓缩。在增生的内膜及其相邻中膜中也发现了凋亡特征，包括凋亡小体的存在。我们的观察结果表明，凋亡和坏死均发生在狭窄的主动脉冠状动脉大隐静脉旁路移植血管的非动脉粥样硬化区域。
