Cherian S M, Bobryshev Y V, Inder S J, Lord R S, Reddi K H, Farnsworth A E, Tran D, Munro V F, Ashwell K W
Surgical Professional Unit, St Vincent's Hospital, Darlinghurst, NSW, Australia.
Cardiovasc Surg. 1999 Aug;7(5):508-18. doi: 10.1016/s0967-2109(99)00021-6.
Antigen-presenting dendritic cells are present in atherosclerotic lesions in human arterial intima, but have not been investigated in atherosclerotic and hyperplastic stenotic lesions that affect vein grafts used as arterial conduits. This study was undertaken to examine whether dendritic cells are present in aortocoronary artery saphenous vein bypass grafts affected by high-grade atheromatous stenosis. Stenotic saphenous vein coronary artery bypass grafts (angiographic luminal stenosis > 75%) were harvested from 10 patients (nine male, one female), aged 4271 years (mean 56.5) at re-do operation. The mean time interval from bypass surgery to the excision of stenotic grafts was 11.5 years (range 2-21). The specimens were fixed in 10% buffered formalin, embedded in paraffin blocks and the sections stained with antibodies to S-100 (to identify dendritic cells), CD3 (T cells), CD68 (macrophages), von Willebrand factor (endothelial cells) and alpha-smooth muscle actin (smooth muscle cells) using avidin-biotin complex immunoperoxidase technique. Normal veins were obtained during saphenous vein femoro-popliteal grafting. The stenotic venous grafts showed histological features typical of extensive arterialization, intimal hyperplasia, atherosclerotic plaque-like lesions, calcification and thrombosis. In areas of intimal hyperplasia, S-10O-positive cells were distributed irregularly among smooth muscle cells. S-100-positive dendritic cells were seen most frequently within atherosclerotic plaque-like lesions where they co-localized with CD3+ cells and CD68+ cells. S-100-positive dendritic cells were also seen accumulating within calcific foci. No S-100-positve cells were found in normal, ungrafted saphenous veins. We conclude that dendritic cells are present in aortocoronary saphenous vein bypass grafts affected by high grade stenosis. Dendritic cells are probably involved in immune mechanisms of atherogenesis through their interactions with T cells and macrophages. The accumulation of dendritic cells within calcific foci suggests their contribution to the calcification of stenotic venous grafts.
抗原呈递树突状细胞存在于人类动脉内膜的动脉粥样硬化病变中，但尚未在影响用作动脉导管的静脉移植物的动脉粥样硬化和增生性狭窄病变中进行研究。本研究旨在检查树突状细胞是否存在于受严重动脉粥样硬化狭窄影响的主动脉冠状动脉大隐静脉旁路移植物中。从10例患者（9例男性，1例女性）身上采集狭窄的大隐静脉冠状动脉旁路移植物（血管造影管腔狭窄>75%），这些患者年龄在42 - 71岁（平均56.5岁），接受再次手术。从旁路手术到切除狭窄移植物的平均时间间隔为11.5年（范围2 - 21年）。标本用10%缓冲福尔马林固定，包埋在石蜡块中，切片用抗S - 100（用于识别树突状细胞）、CD3（T细胞）、CD68（巨噬细胞）、血管性血友病因子（内皮细胞）和α - 平滑肌肌动蛋白（平滑肌细胞）的抗体，采用抗生物素蛋白 - 生物素复合物免疫过氧化物酶技术进行染色。在大隐静脉股 - 腘动脉移植术中获取正常静脉。狭窄的静脉移植物呈现出广泛动脉化、内膜增生、动脉粥样硬化斑块样病变、钙化和血栓形成的典型组织学特征。在内膜增生区域，S - 100阳性细胞不规则地分布在平滑肌细胞之间。S - 100阳性树突状细胞最常见于动脉粥样硬化斑块样病变内，它们与CD3 + 细胞和CD68 + 细胞共定位。S - 100阳性树突状细胞也见于钙化灶内聚集。在正常的、未移植的大隐静脉中未发现S - 100阳性细胞。我们得出结论，树突状细胞存在于受严重狭窄影响的主动脉冠状动脉大隐静脉旁路移植物中。树突状细胞可能通过与T细胞和巨噬细胞的相互作用参与动脉粥样硬化的免疫机制。树突状细胞在钙化灶内的聚集表明它们对狭窄静脉移植物钙化的作用。
