Bechara I J, Joazeiro P P, Marí-Beffa M, Becerra J, Montes G S
Department of Histology and Embryology, Institute of Biology, UNICAMP, Campinas, SP, Brazil.
J Submicrosc Cytol Pathol. 2000 Apr;32(2):273-80.
Regenerating tail fins were studied in two species of teleosts, Tilapia rendalli and Cyprinus carpio, treated with indomethacin, aspirin, dexamethasone, penicillamine, and beta-aminoproprionitrile, drugs known to disrupt collagen metabolism in mammals. Collagen was studied under the light microscope by the Picrosirius-polarization method and also under the electron microscope. In general, these drugs disturbed the deposition and organization of collagen fibrils leading to abnormally thin or practically absent lepidotrichia and actinotrichia, and also to disorganized fibrous connective tissue. The resulting disorganization of the collagenous scaffolding of the regenerating dermoskeleton was probably responsible for a secondary effect on blastema distalization and on the general fin ray patterning that were also observed. The foregoing observations suggest that the stromal histoarchitecture of the regenerate plays a vital role in fin regeneration and indicate that these drugs may be useful in studying the extracellular matrix-cell interactions at the cellular and molecular level. In addition, the present findings provide a basis for developing different biological models by using teleost fin regeneration.
在罗非鱼（Tilapia rendalli）和鲤鱼（Cyprinus carpio）这两种硬骨鱼中，研究了用消炎痛、阿司匹林、地塞米松、青霉胺和β-氨基丙腈处理后的再生尾鳍。这些药物在哺乳动物中已知会破坏胶原蛋白代谢。通过天狼星苦味酸偏振法在光学显微镜下以及在电子显微镜下对胶原蛋白进行了研究。一般来说，这些药物扰乱了胶原纤维的沉积和组织，导致鳞质鳍条和鳍条骨异常纤细或几乎缺失，还导致纤维结缔组织紊乱。再生真皮骨骼的胶原支架结构紊乱可能是对芽基远端化和一般鳍条模式产生的次要影响的原因，这些影响也被观察到。上述观察结果表明，再生组织的基质组织架构在鳍再生中起着至关重要的作用，并表明这些药物可能有助于在细胞和分子水平上研究细胞外基质与细胞的相互作用。此外，目前的研究结果为利用硬骨鱼鳍再生开发不同的生物学模型提供了基础。
