Huen D S, Elsdon M, Ponder B A
Department of Genetics, University of Cambridge, UK.
Mol Gen Genet. 2000 Oct;264(3):335-40. doi: 10.1007/s004380000327.
We have cloned and sequenced the 5' and 3' ends of the Drosophila homolog of the vertebrate c-ret gene, Ret, and have derived from it the predicted protein sequence of Ret. The extracellular domain of Ret is very widely diverged from that of its vertebrate counterparts but the cadherin motif present in vertebrate c-ret proteins can also be discerned in Ret. As with the vertebrate gene, multiple splice variants were detected at the 5'-end of Ret, one of which inserts an exon with a protein-terminating frameshift into the cDNA. In contrast to human c-ret, which may vary its signalling specificity by using splicing-derived, alternative C-terminal sequences, Ret cDNAs showed no variation at their 3'-ends.
我们已经克隆并测序了脊椎动物c-ret基因的果蝇同源基因Ret的5'端和3'端，并从中推导了Ret的预测蛋白质序列。Ret的细胞外结构域与其脊椎动物对应物的差异非常大，但脊椎动物c-ret蛋白中存在的钙黏蛋白基序在Ret中也可以识别。与脊椎动物基因一样，在Ret的5'端检测到多个剪接变体，其中一个将带有蛋白质终止移码的外显子插入cDNA中。与人类c-ret不同，人类c-ret可能通过使用剪接衍生的替代C端序列来改变其信号特异性，而Ret cDNA在其3'端没有变化。
