16型人乳头瘤病毒在宫颈肿瘤中的整合经常发生在常见易碎位点。

Human papillomavirus type 16 integrations in cervical tumors frequently occur in common fragile sites.

作者信息

Thorland E C, Myers S L, Persing D H, Sarkar G, McGovern R M, Gostout B S, Smith D I

机构信息

Department of Biochemistry, Mayo Clinic, Rochester, Minnesota 55905, USA.

出版信息

Cancer Res. 2000 Nov 1;60(21):5916-21.

PMID:11085503

Abstract

The development of cervical cancer is highly associated with human papillomavirus (HPV) infection. HPV integration into the genome of infected cervical cells is temporally associated with the acquisition of the malignant phenotype. A relationship between the sites of HPV integration in cervical cancer and the position of the common fragile sites (CFSs) has been observed at the cytogenetic level. To explore this relationship at the molecular level, we used a PCR-based method to rapidly isolate cellular sequences flanking the sites of HPV16 integrations in primary cervical tumors. Human bacterial artificial chromosome clones were isolated based on these flanking sequences and used as probes for fluorescence in situ hybridization on metaphases derived from cells cultured in the presence of aphidicolin. Our data demonstrate that HPV16 integrations in cervical tumors frequently occur within CFSs at the molecular level. In addition, we have determined the precise molecular locations of the CFSs FRA6C and FRA17B.

摘要

宫颈癌的发展与人类乳头瘤病毒（HPV）感染高度相关。HPV整合到受感染宫颈细胞的基因组中与恶性表型的获得在时间上相关。在细胞遗传学水平上，已观察到宫颈癌中HPV整合位点与常见脆性位点（CFSs）位置之间的关系。为了在分子水平上探究这种关系，我们使用基于聚合酶链反应（PCR）的方法快速分离原发性宫颈肿瘤中HPV16整合位点两侧的细胞序列。基于这些侧翼序列分离出人类细菌人工染色体克隆，并将其用作在阿非科林存在下培养的细胞衍生的中期染色体上进行荧光原位杂交的探针。我们的数据表明，在分子水平上，宫颈肿瘤中的HPV16整合经常发生在CFSs内。此外，我们已经确定了CFSs FRA6C和FRA17B的精确分子位置。