Langlands K, Down G A, Kealey T
University of Cambridge, Department of Clinical Biochemistry, Addenbrooke's Hospital, United Kingdom.
Cancer Res. 2000 Nov 1;60(21):5929-33.
Helix-loop-helix genes regulate many developmental pathways, and growing evidence associates dysregulated expression with tumorigenesis. We observed Id-1, Id-2, and Id-3 mRNA expression in proliferating human keratinocytes in vitro with subsequent down-regulation with differentiation. Immunohistochemical analysis of human tissue sections identified cytoplasmic Id-1 expression and nuclear Id-2 and Id-3 expression in the proliferating layers of the epidermis. Furthermore, we observed a columnar pattern of Id-2 and Id-3 staining, which may relate to the epidermal proliferative unit. In squamous cell carcinoma of the head and neck, Id protein immunoreactivity was observed in the majority of malignant keratinocytes in the most poorly differentiated sections, with reduced staining in well-differentiated disease.
螺旋-环-螺旋基因调控许多发育途径，越来越多的证据表明其表达失调与肿瘤发生有关。我们观察了体外增殖的人角质形成细胞中Id-1、Id-2和Id-3 mRNA的表达，随后随着分化其表达下调。对人体组织切片的免疫组织化学分析表明，在表皮的增殖层中，Id-1在细胞质中表达，Id-2和Id-3在细胞核中表达。此外，我们观察到Id-2和Id-3染色呈柱状模式，这可能与表皮增殖单位有关。在头颈部鳞状细胞癌中，在分化最差的切片中，大多数恶性角质形成细胞中观察到Id蛋白免疫反应性，而在高分化病变中染色减少。
