Gorelick R J, Lifson J D, Yovandich J L, Rossio J L, Piatak M, Scarzello A J, Knott W B, Bess J W, Fisher B A, Flynn B M, Henderson L E, Arthur L O, Benveniste R E
AIDS Vaccine program, SAIC-Frederick, National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, MD 21702-1201, USA.
J Med Primatol. 2000 Aug;29(3-4):209-19. doi: 10.1034/j.1600-0684.2000.290314.x.
A simian immunodeficiency virus (SIV)(Mne) DNA clone was constructed that produces viruses containing a four amino acid deletion in the second zinc finger of the nucleocapsid (NC) domain of the Gag polyprotein. Viruses produced from this clone, although non-infectious both in vitro and in vivo, complete a majority of the steps in a single retroviral infection cycle. Eight pig-tailed macaques (Macaca nemestrina) were inoculated intramuscularly and subcutaneously three times over the course of 24 weeks with the NC mutant expressing DNA. These macaques, and four controls, were then challenged mucosally (intrarectally) with the homologous virus (SIV Mne CL E11S) and monitored for evidence of infection and clinical disease. Prior to challenge, a measurable humoral immune response was noted in four of eight immunized macaques. After challenge, all 12 macaques became infected, although four immunized animals greatly restricted their viral replication, and one immunized animal that controlled replication remains antibody negative. No disease has been evidence during the 46-week period of monitoring after challenge.
构建了一种猿猴免疫缺陷病毒（SIV）（Mne）DNA克隆，该克隆产生的病毒在Gag多聚蛋白核衣壳（NC）结构域的第二个锌指中存在四个氨基酸缺失。从该克隆产生的病毒虽然在体外和体内均无感染性，但在单个逆转录病毒感染周期中可完成大部分步骤。在24周的时间里，对8只食蟹猴（Macaca nemestrina）进行了三次肌肉内和皮下接种，接种的是表达NC突变体的DNA。然后，对这些食蟹猴和4只对照动物进行黏膜（直肠内）同源病毒（SIV Mne CL E11S）攻击，并监测感染和临床疾病的证据。在攻击前，8只免疫的食蟹猴中有4只出现了可测量的体液免疫反应。攻击后，所有12只食蟹猴均被感染，尽管4只免疫动物的病毒复制受到极大限制，且有1只控制病毒复制的免疫动物仍为抗体阴性。在攻击后的46周监测期内未发现疾病迹象。
